As all kinds of electronic products tend to be miniaturized, the application of traditional welding technology in various new electronic components has a certain test. In order to meet such market demand, in the welding technology, it can be said that the technology is constantly improving, and the welding way is more diversified. The Light Source for laser welding is a laser light-emitting diode, which can be focused on the solder spot by an optical system. The advantage of laser welding is that it can precisely control and optimize the energy required for welding. It is suitable for selective reflow welding process or using tin wire connector. For SMD components, the solder paste should be applied first and then welded. The welding process is divided into two steps: first the solder paste is heated, and the solder joint is preheated. After that, the solder paste used for welding is completely melted, and the solder is completely wetted on the pad to form the weld. Use laser generator and optical focusing component welding, energy density, high heat transfer efficiency, non-contact welding, solder can be solder paste or tin wire, especially suitable for welding small space solder spot or small solder spot power is small, save energy. Laser welding features: ● Multi-axis servo motor board card control, high positioning accuracy ● Small laser spot, in the small size of pads, spacing devices have obvious welding advantages ● Non-contact welding, no mechanical stress, static risk ● Wuxi slag, reduce flux waste, low production cost ● Weldable products are rich in type ● Choice of solder Advantages of laser welding: For ultra-fine electronic substrate, multi-layer electronic components, "traditional technology" has been unable to apply, which prompted the rapid progress of technology. The processing of ultra-small parts that are not suitable for the traditional soldering iron method is finally completed by laser welding. Non - contact welding is the biggest advantage of laser welding. There is no need to touch the substrate and electronic components at all, and providing solder only by laser irradiation does not pose a physical burden. Effective heating with a blue laser beam is also an advantage, which can be used to illuminate narrow areas where the soldering head cannot enter and at different angles when there is no distance between adjacent components in a dense assembly. The soldering iron head needs to be replaced regularly, while the spare parts for laser welding need to be replaced are very few and the maintenance cost is low. Hecho Technology supply the Fiber Optic Bundles for Laser selective soldering, customized service is available to meet the different design requirements of customers.

Dear Customers, Sincerely thanks for your always support. The traditional Mid-Autumn Festival and China National Day is coming, wish you all a happy holiday. Pls note that Hecho Fiber Optics will have couple days holiday for the two holiday: Mid-Autumn Festival: from Sep. 19th to Sep. 21st National Day: from Oct. 1st to Oct. 7th If there's any new questions about the Hecho Fiber Optic Bundles & Fiber Optic Illuminator, pls send E-mail to owen@gohecho.cn or any emergency pls call to +86 133 8200 9944. Keep Social distancing, pay attention to personal protection for the COVID-19. Thanks & best regards, Nanjing Hecho Technology Co., Ltd 2021-9-8

Dear Value Customers, Sincerely thanks for your always support. The traditional Dragon Boat Festival is coming, wish you all a happy holiday. Pls note that Hecho Technology will have a three days holiday from June 22 to June 24. If there's any new inquiry about the customized Fiber Optic & Light Source, pls send E-mail to owen@gohecho.cn or any emergency pls call to +86 133 8200 9944. Keep Social distancing, pay attention to personal protection for the COVID-19. Thanks & best regards, Nanjing Hecho Technology Co., Ltd. 2023-6-02

The difference between quantitative real-time PCR (qPCR) and end-point PCR is the step at which the PCR product amount is measured. In traditional PCR, product quantitation is performed once at the end of the reaction. In qPCR, the amount of PCR product is measured after every amplification round. qPCR instruments are designed for both PCR cycling and the detection of signal from fluorescent dyes during amplification. By monitoring the progress of amplification in real time, the point in time when the amplification of the target is detected above background can be identified. The fewer targets present in the reaction, the later the first significant signal is detected. This technique, therefore, is of great advantage when gene-expression differences are analyzed. There are two ways to detect PCR signal with the help of fluorescent dyes: fluorescent dyes that nonspecifically bind to double-stranded DNA (Dye-based qPCR) or fluorescently labeled, sequence-specific probes (Probe-based qPCR). In Dye-based qPCR the use of universal fluorescent DNA-intercataling dyes enables analysis of many targets without having to synthesize sequence-specific probes. However, this method has the disadvantage of detecting all specific and unspecific amplification events as well as primer–dimers because the dye binds to all double-stranded DNA. To achieving high PCR specificity, avoiding background and primer–dimer formation is critical. Probe-based qPCR method is more precise, as the use of sequence-specific fluorescently labeled probes ensures the detection of the target product only. A variety of probe fluorescence chemistry is currently available. Multiple fluorescent probes can be used in multiplex qPCR, although the use of specific probes is more expensive than using DNA-intercalating dyes. qRT-PCR One Step Kits allow reverse transcription and real-time PCR to be performed in one step. In this case, RNA is the template for qPCR. One-step qPCR kits include separate qPCR and reverse transcription master mixes. Same like qPCR, the qRT-PCR One Step Kits are also available in both Dye-based and Probe-based formats. Many real-time PCR instruments available on the market require a passive internal reference dye (either ROX™ or fluorescein) for normalization of the fluorescent signal. The use of passive reference dyes does not interfere with the qPCR. The reference dyes are either supplied separately, or they can be included in the qPCR mastermix at appropriate concentration. biotechrabbit offers both. The choice depends on your instrument requirements for reference dye.

Nanjing Hecho Technology is a leading provider of Fiber Optic solutions in China since 2004. By providing innovative technology and product solutions to customers in multiple countries around the world, companies and customers can enjoy the value of innovation. Products including Fiber Optic Bundle, Fiber Optic Illuminator, Fiber Optic Sensor, and so on, flexible to meet the diverse needs of customers in different industries, such as industry, medical, lighting, optics, and the pursuit of rapid innovation. The Laser and Photonics Exhibition will be held from March 17th to 19th, 2021. Our booth number is W5.5155. Welcome every customers to visit our booth!

Dear valued customers, 2020 was an extraordinary year, People all over the world are still suffering from the epidemic COVID-19. I hope we can win the fight against the epidemic soon. In order to better serve your company, we hereby inform you of our company's holiday time for the Chinese New Year in 2021, so as to make good preparations for the holidays. Thanks for your support! Pls note that Hecho will be closed for the CNY celebration from Feb. 10th 2021 to Feb. 19th 2021. Normal business will resume on Feb. 20th 2021. During the holiday period, there will likely be a delay in reply to your email or phone call, we apologize for the delay and any inconvenience this may cause. If something urgent comes up or you need any immediate help, pls feel free ti reach out to us via email and call. If there's any questions on Fiber Optic Illuminators or Fiber Optic Bundles and Fiber Optic Cables, pls feel free to contact us. Best Wishes. Nanjing Hecho Technology Co., Ltd 2021-2-4

Analytica China in Munich is Asia's largest international expo in the field of analysis and biochemical technology. It is a platform for outstanding companies in the industry to comprehensively display new technologies, products and solutions. Bringing together industry enterprises, showing front-end technology and high-end products, is the vane of industry development. analytica China will comprehensively cover and display various fields of laboratory research and industry development: Life Science, Biotechnology and Diagnosis Analysis and quality control Laboratory equipment and technology Food safety equipment and technology Environmental monitoring equipment and technology Due to the sudden outbreak of the new crown virus, the biochemical analysis industry market is extremely hot. Taking this opportunity, Nanjing Hongzhao Technology Co., Ltd. will exhibit a variety of High transfer rate and Low CV value Fiber Optic Bundles, PCR Fiber Optic Cables that can be used in nucleic acid detection equipment. Welcome new and old customers of Everbright to visit and guide. Exhibition time: November 16-18, 2020 Pavilion: Shanghai New International Expo Center Booth number: E5. 5211

Dear customers, According to the national statutory holiday regulations and combined with the actual situation of the company, the arrangements for the National Day and Mid-Autumn Festival holidays in 2020 are as follows: 1. There will be a holiday from October 1 to 8, 2020, for a total of 8 days. Work on October 9 (Friday). 2. All employees of the company are requested to make their own pre-holiday work arrangements, and check related facilities and equipment, do a good job in fire prevention and theft, and ensure the safety and order of the workshop and office. 3. The employees of the company should maintain smooth communication during the holidays to meet the needs of the company's work. During the holiday season, everyone pays attention to safety. The epidemic is not over yet, so you must wear a mask when you go out. 4. I wish our customers and all employees a happy holiday and good health in advance! If there's any requirements for the Fiber Optic Illuminators, Fiber Optic Bundles, and other Fiber Optic products in this period, pls E-mail to: owen@gohecho.cn. NANJING HECHO TECHNOLOGY CO., LTD. 2020-9-28

Nanjing Hecho Technology is a leading provider of Fiber Optic solutions in China since 2004. By providing innovative technology and product solutions to customers in multiple countries around the world, companies and customers can enjoy the value of innovation. Products including Fiber Optic Bundles, Fiber Optic Illuminators, Fiber Optic Sensors, Machine Vision Lighting and so on, flexible to meet the diverse needs of customers in different industries, such as industry, medical, lighting, optics, and the pursuit of rapid innovation. Affected by the COVID-19, The CIOE exhibition will be held from September 9th to 11th, 2020. Our booth number is 5E50. Welcome every customers to visit our booth!

LASER WORLD OF PHOTONICS CHINA presents the full range of product content of the optoelectronic industry from an international perspective, designed to meet the unique needs of the Chinese market. As the Asian laser, optics, and optoelectronics industry event, from basic research to industrial applications, the Munich Shanghai Optical Expo leads the industry development trend, focusing on covering lasers and optoelectronics, optical and optical manufacturing, laser production and processing technology, imaging, inspection and quality control The comprehensive product content of the four major sectors is an industry exhibition not to be missed. Affected by this year's new coronavirus, this Expo will be postponed to July 3 to 5, Nanjing Hecho Technology Co., Ltd. will continue to participate in this Shanghai Expo in Munich, welcome friends to visit. In this exhibition, Nanjing Hecho Technology will exhibit traditional superior products such as Fiber Optic Bundles, near-infrared optical fiber light guide, Fiber Optic Illuminator, including Halogen light source, LED light source, etc. As an authorized agent of SCHOTT Group, our company will also exhibit SCHOTT cold light sources and fiber optic bundles. In addition, the company's newly developed high-power, ultra-high brightness LED cold light source will have the opportunity to exhibit. Nanjing Hecho Technology will provide you with more professional optical fiber transmission solutions. Our booth is at 7.1A 143. Welcome!

Nucleic acid detection principle of a new coronavirus All organisms contain nucleic acids other than prions. Nucleic acids include deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). The new coronavirus is a virus that contains only RNA. Specific RNA sequences in the virus distinguish the virus from other pathogens Landmarks. After the emergence of the new coronavirus, Chinese scientists completed the analysis of the whole genome sequence of the new coronavirus in a very short time, and compared the genome sequences of other species with the specific nucleic acid sequences in the new coronavirus. During clinical laboratory testing, if the specific nucleic acid sequence of a novel coronavirus can be detected in a patient sample, it should be indicated that the patient may be infected with the novel coronavirus. The most common method for detecting novel coronavirus-specific sequences is real-time PCR (polymerase chain reaction). Because the PCR reaction template is only DNA, the new coronavirus nucleic acid (RNA) should be reverse transcribed into DNA before the PCR reaction. In the PCR reaction system, a pair of specific primers and a Taqman probe are included. The probe is a specific oligonucleotide sequence, and both ends are labeled with a reporter fluorescent group and a quenching fluorescent group. When the probe is complete, the fluorescent signal emitted by the reporter group is absorbed by the quenching group; if a target sequence exists in the reaction system, the probe is bound to the template during the PCR reaction, and the DNA polymerase uses the enzyme's exonuclease activity along the template to Enzymatic digestion degrades, the reporter group is separated from the quenching group, and emits fluorescence. For each DNA strand amplified, a fluorescent molecule is produced. The quantitative PCR instrument can monitor that the number of cycles (Ct value) at which the fluorescence reaches a preset threshold is related to the viral nucleic acid concentration. The higher the viral nucleic acid concentration, the smaller the Ct value. The products of different manufacturers will determine the positive judgment value of this product according to the performance of their own products. In recent years, Hecho company has supplied high performance Fiber Optic Cable and Fiber Optic Bundles to more and more nucleic acid detection manufacturers. The customized optical fiber completely meets the requirements of customers and has won unanimous praise from the market.

Dear customer, Thank you for your always support and pleasant cooperation in our work in the past year. In order to better serve your company, we hereby inform you of our company's holiday time for the New Year and Spring Festival in 2020, so as to make good preparations for the holidays. Thanks for your support! Holiday arrangement: New Year: Jan. 1st, 2020. Spring Festival: Jan. 20, 2020 Until to Feb. 2, 2020. If there's any questions on Fiber Optic Illuminator or Light Guides and Fiber Optic Cables, pls feel free to contact us. Best Wishes. Nanjing Hecho Technology Co., Ltd 2019-12-24